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entry vectors pucl3l2  (Addgene inc)


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    Addgene inc entry vectors pucl3l2
    Entry Vectors Pucl3l2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/entry+vectors+pucl3l2/pUC-L3L2+(Plasmid+%23114019)/pm39833163-195-17-22
    Average 93 stars, based on 4 article reviews
    entry vectors pucl3l2 - by Bioz Stars, 2026-09
    93/100 stars

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    Amplification:

    Article Title: Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis.
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid#114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc2in1-NC40 (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_ + _35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_ + _35S::BEN1-mCherry.

    Article Title: Implementation of pupylation-based proximity labelling in plant biology reveals regulatory factors in cellulose biosynthesis
    Article Snippet: .. The coding sequences of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors PUCL3L2 (Plasmid #114019, addgene) and PUCL1L4 (Plasmid #114018, addgene) respectively. .. Then BEN1, CC1 and BEN1 were introduced into the pFRETgc-2in1-NC (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S:: EGFP-CC1 + 35S::BEN1-mCherry. pFRETgc 35S::EGFP + 35S::BEN1-mCherry were co-expressed with pMDC32_35S::FLAG-Pup(E) _+_UBIpro::::PafA-CC1 or not in tobacco leaves, then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S:: EGFP-CC1 + 35S::BEN1-mCherry were expressed in tobacco leaves respectively, then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).

    Article Title: Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid #114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc-2in1-NC (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_ + _35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_ + _35S::BEN1-mCherry.

    Polymerase Chain Reaction:

    Article Title: Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis.
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid#114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc2in1-NC40 (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_ + _35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_ + _35S::BEN1-mCherry.

    Article Title: Implementation of pupylation-based proximity labelling in plant biology reveals regulatory factors in cellulose biosynthesis
    Article Snippet: .. The coding sequences of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors PUCL3L2 (Plasmid #114019, addgene) and PUCL1L4 (Plasmid #114018, addgene) respectively. .. Then BEN1, CC1 and BEN1 were introduced into the pFRETgc-2in1-NC (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S:: EGFP-CC1 + 35S::BEN1-mCherry. pFRETgc 35S::EGFP + 35S::BEN1-mCherry were co-expressed with pMDC32_35S::FLAG-Pup(E) _+_UBIpro::::PafA-CC1 or not in tobacco leaves, then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S:: EGFP-CC1 + 35S::BEN1-mCherry were expressed in tobacco leaves respectively, then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).

    Article Title: Pupylation-based proximity labelling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were ampli ed by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid #114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc-2in1-NC 41 (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_+_35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_+_35S::BEN1-mCherry.

    Article Title: Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid #114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc-2in1-NC (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_ + _35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_ + _35S::BEN1-mCherry.

    Article Title: Pupylation-based proximity labelling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were ampli ed by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid #114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc-2in1-NC 41 (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_+_35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_+_35S::BEN1-mCherry.

    Plasmid Preparation:

    Article Title: Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis.
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid#114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc2in1-NC40 (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_ + _35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_ + _35S::BEN1-mCherry.

    Article Title: Implementation of pupylation-based proximity labelling in plant biology reveals regulatory factors in cellulose biosynthesis
    Article Snippet: .. The coding sequences of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors PUCL3L2 (Plasmid #114019, addgene) and PUCL1L4 (Plasmid #114018, addgene) respectively. .. Then BEN1, CC1 and BEN1 were introduced into the pFRETgc-2in1-NC (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S:: EGFP-CC1 + 35S::BEN1-mCherry. pFRETgc 35S::EGFP + 35S::BEN1-mCherry were co-expressed with pMDC32_35S::FLAG-Pup(E) _+_UBIpro::::PafA-CC1 or not in tobacco leaves, then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S:: EGFP-CC1 + 35S::BEN1-mCherry were expressed in tobacco leaves respectively, then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).

    Article Title: Pupylation-based proximity labelling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were ampli ed by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid #114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc-2in1-NC 41 (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_+_35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_+_35S::BEN1-mCherry.

    Article Title: Pupylation-based proximity labeling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were amplified by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid #114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc-2in1-NC (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_ + _35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_ + _35S::BEN1-mCherry.

    Article Title: Pupylation-based proximity labelling reveals regulatory factors in cellulose biosynthesis in Arabidopsis
    Article Snippet: .. The CDS of CC1 and BEN1 (without stop codon) were ampli ed by PCR and subcloned into the entry vectors pUCL3L2 (Plasmid #114019, addgene) and pUCL1L4 (Plasmid #114018, addgene) respectively. .. Then pUCL1L4_BEN1 and pUCL3L2_CC1 were introduced into the pFRETgc-2in1-NC 41 (Plasmid #105121, addgene) vector by LR reaction to generate the pFRETgc_35S::EGFP_+_35S::BEN1-mCherry and pFRETgc_35S::EGFP-CC1_+_35S::BEN1-mCherry.



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    entry vectors pucl3l2  (Addgene inc)
    93
    Addgene inc entry vectors pucl3l2
    Entry Vectors Pucl3l2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/entry+vectors+pucl3l2/pUC-L3L2+(Plasmid+%23114019)/pm39833163-195-17-22
    Average 93 stars, based on 1 article reviews
    entry vectors pucl3l2 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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